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수정능력 획득 후 고수태성 정자와 저수태성 정자에서 차등 발현하는 유전자의 발현을 확인할 수 있는 제제를 포함하는 것을 특징으로 하는, 수컷 돼지의 수태능력 예측을 위한 전사체 바이오 마커 조성물로서,상기 유전자는 TMSB4X(thymosin beta 4, X-linked)인 것을 특징으로 하는, 조성물
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삭제
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삭제
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제1항에 있어서,상기 조성물은 PLEK(pleckstrin), CD53(CD53 molecule), ABCC1(ATP Binding Cassette Subfamily C Member 1), RAB7L1(RAB7, member RAS oncogene family-like 1), MCMBP(minichromosome maintenance complex binding protein), CRIM1(cysteine rich transmembrane BMP regulator 1), TAF12(TATA-box binding protein associated factor 12), PTPRC(protein tyrosine phosphatase, receptor type C), STRIP2(striatin interacting protein 2), DGCR8(microprocessor complex subunit DGCR8), ASXL2(additional sex combs like 2), RAB11FIP2(RAB11 family interacting protein 2), JARID2(jumonji and AT-rich interaction domain containing 2), CXCR4(C-X-C motif chemokine receptor 4), DUSP2(dual specificity phosphatase 2), RAB11B(Ras-related protein Rab-11B), LCP1(lymphocyte cytosolic protein 1), SLA-DQA1(MHC class II, DQ alpha 1), HOMER2(homer scaffolding protein 2), ANO6(anoctamin 6), EPHB2(EPH receptor B2), CELF6(CUGBP, Elav-like family member 6), EGR2(early growth response 2), CNPY1(canopy FGF signaling regulator 1), MYL6(myosin light chain 6), GANAB(glucosidase II alpha subunit), RMDN1(regulator of microtubule dynamics 1), GIPC2(GIPC PDZ domain containing family member 2), MS4A2(membrane spanning 4-domains A2), BUB3(mitotic checkpoint protein BUB3), ITGA6(integrin subunit alpha 6), FMR1(fragile X mental retardation 1), GXYLT2(glucoside xylosyltransferase 2), PFDN6(prefoldin subunit 6), RPS28(ribosomal protein S28), RPS20(ribosomal protein S20), SSR4(signal sequence receptor subunit 4), SGK1(serum/glucocorticoid regulated kinase 1), SORD(sorbitol dehydrogenase), ZDHHC2(zinc finger DHHC-type containing 2), MDK(midkine), NFKBIA(NF-κB inhibitor alpha), TMEM170B(transmembrane protein 170B), KLF6(kruppel like factor 6), TRIM59(tripartite Motif Containing 59), CYFIP2(cytoplasmic FMR1 interacting protein 2), UTP15(U3 small nucleolar RNA-associated protein 15), ST13(suppression of tumorigenicity 13), 및 ARPC4(actin related protein 2/3 complex subunit 4)로 이루어진 군에서 선택되는 하나 이상의 유전자의 발현을 확인할 수 있는 제제를 더 포함하는 것을 특징으로 하는, 수컷 돼지의 수태능력 예측을 위한 전사체 바이오 마커 조성물
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수정능력 획득 후 고수태성 정자와 저수태성 정자에서 차등 발현하는 유전자의 mRNA에 상보적으로 결합하는 센스 프라이머와 안티센스 프라이머를 포함하는 프라이머 세트, 또는 프로브를 포함하는, 수컷 돼지의 수태능력 예측용 키트로서,상기 유전자는 TMSB4X(thymosin beta 4, X-linked)인 것을 특징으로 하는, 키트
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삭제
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제5항에 있어서,상기 유전자는 PLEK(pleckstrin), CD53(CD53 molecule), ABCC1(ATP Binding Cassette Subfamily C Member 1), RAB7L1(RAB7, member RAS oncogene family-like 1), MCMBP(minichromosome maintenance complex binding protein), CRIM1(cysteine rich transmembrane BMP regulator 1), TAF12(TATA-box binding protein associated factor 12), PTPRC(protein tyrosine phosphatase, receptor type C), STRIP2(striatin interacting protein 2), DGCR8(microprocessor complex subunit DGCR8), ASXL2(additional sex combs like 2), RAB11FIP2(RAB11 family interacting protein 2), JARID2(jumonji and AT-rich interaction domain containing 2), CXCR4(C-X-C motif chemokine receptor 4), DUSP2(dual specificity phosphatase 2), RAB11B(Ras-related protein Rab-11B), LCP1(lymphocyte cytosolic protein 1), SLA-DQA1(MHC class II, DQ alpha 1), HOMER2(homer scaffolding protein 2), ANO6(anoctamin 6), EPHB2(EPH receptor B2), CELF6(CUGBP, Elav-like family member 6), EGR2(early growth response 2), CNPY1(canopy FGF signaling regulator 1), MYL6(myosin light chain 6), GANAB(glucosidase II alpha subunit), RMDN1(regulator of microtubule dynamics 1), GIPC2(GIPC PDZ domain containing family member 2), MS4A2(membrane spanning 4-domains A2), BUB3(mitotic checkpoint protein BUB3), ITGA6(integrin subunit alpha 6), FMR1(fragile X mental retardation 1), GXYLT2(glucoside xylosyltransferase 2), PFDN6(prefoldin subunit 6), RPS28(ribosomal protein S28), RPS20(ribosomal protein S20), SSR4(signal sequence receptor subunit 4), SGK1(serum/glucocorticoid regulated kinase 1), SORD(sorbitol dehydrogenase), ZDHHC2(zinc finger DHHC-type containing 2), MDK(midkine), NFKBIA(NF-κB inhibitor alpha), TMEM170B(transmembrane protein 170B), KLF6(kruppel like factor 6), TRIM59(tripartite Motif Containing 59), CYFIP2(cytoplasmic FMR1 interacting protein 2), UTP15(U3 small nucleolar RNA-associated protein 15), ST13(suppression of tumorigenicity 13), 및 ARPC4(actin related protein 2/3 complex subunit 4)로 이루어진 군에서 선택되는 하나 이상의 유전자를 더 포함하는 것을 특징으로 하는, 수컷 돼지의 수태능력 예측용 키트
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(1) 개체로부터 분리한 수정능력 획득 후의 고수태성 정자와 저수태성 정자에서 차등 발현하는 유전자의 mRNA 발현량을 측정하는 단계; 및(2) 상기 mRNA 발현량이 수태능력에 따라 증가 또는 감소하는 것을 분석하는 단계를 포함하는, 수컷 돼지의 수태능력 예측 또는 진단 방법으로서,상기 (1) 단계의 유전자는 TMSB4X(thymosin beta 4, X-linked)인 것을 특징으로 하는, 방법
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삭제
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삭제
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제9항에 있어서,상기 (1) 단계의 유전자는 PLEK(pleckstrin), CD53(CD53 molecule), ABCC1(ATP Binding Cassette Subfamily C Member 1), RAB7L1(RAB7, member RAS oncogene family-like 1), MCMBP(minichromosome maintenance complex binding protein), CRIM1(cysteine rich transmembrane BMP regulator 1), TAF12(TATA-box binding protein associated factor 12), PTPRC(protein tyrosine phosphatase, receptor type C), STRIP2(striatin interacting protein 2), DGCR8(microprocessor complex subunit DGCR8), ASXL2(additional sex combs like 2), RAB11FIP2(RAB11 family interacting protein 2), JARID2(jumonji and AT-rich interaction domain containing 2), CXCR4(C-X-C motif chemokine receptor 4), DUSP2(dual specificity phosphatase 2), RAB11B(Ras-related protein Rab-11B), LCP1(lymphocyte cytosolic protein 1), SLA-DQA1(MHC class II, DQ alpha 1), HOMER2(homer scaffolding protein 2), ANO6(anoctamin 6), EPHB2(EPH receptor B2), CELF6(CUGBP, Elav-like family member 6), EGR2(early growth response 2), CNPY1(canopy FGF signaling regulator 1), MYL6(myosin light chain 6), GANAB(glucosidase II alpha subunit), RMDN1(regulator of microtubule dynamics 1), GIPC2(GIPC PDZ domain containing family member 2), MS4A2(membrane spanning 4-domains A2), BUB3(mitotic checkpoint protein BUB3), ITGA6(integrin subunit alpha 6), FMR1(fragile X mental retardation 1), GXYLT2(glucoside xylosyltransferase 2), PFDN6(prefoldin subunit 6), RPS28(ribosomal protein S28), RPS20(ribosomal protein S20), SSR4(signal sequence receptor subunit 4), SGK1(serum/glucocorticoid regulated kinase 1), SORD(sorbitol dehydrogenase), ZDHHC2(zinc finger DHHC-type containing 2), MDK(midkine), NFKBIA(NF-κB inhibitor alpha), TMEM170B(transmembrane protein 170B), KLF6(kruppel like factor 6), TRIM59(tripartite Motif Containing 59), CYFIP2(cytoplasmic FMR1 interacting protein 2), UTP15(U3 small nucleolar RNA-associated protein 15), ST13(suppression of tumorigenicity 13), 및 ARPC4(actin related protein 2/3 complex subunit 4)로 이루어진 군에서 선택되는 하나 이상의 유전자를 더 포함하는 것을 특징으로 하는, 수컷 돼지의 수태능력 예측 또는 진단 방법
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